Evaluation of embryo quality after concurrent use of ovarian stimulating hormones and gamma irradiation

Background: radiotherapy has many side effects on fertilization in young women. radiation can lead to ovarian failure in women who underwent abdomen or pelvic radiotherapy. objective: this study helps us to investigate ovarian response of nmri female mice to ovarian stimulating hormones (pmsg, hcg) after whole-body gamma irradiation. materials and methods: 45 pregnant mice were divided into two groups of control and experimental. the experimental group was classified into three sub-groups: irradiation group (2 or 4gy),superovulation group (10 or 15iu),and superovulation and gamma-radiation group (2gy & 10iu, 2gy & 15iu, 4gy & 10iu,4gy & 15iu). female mice were killed and embryos were removed from oviduct .the number of embryos cells counted and the quality of them was evaluated in each group. kruskal-wallis test and mann-whitney test were used to analyze the data. results: there was a significant difference in the number of 2-4 cells grade d embryos in 2gy & 15iu group compared with control and 2gy groups (p=0.01), and the number of embryos in 4gy group was more than in 10iu and 15iu (p=0.03) and 2gy & 15iu groups (p=0.01). it was more significantly embryos in 4gy & 15iu group compared to 2gy & 15iu group (p=0.01).in addition there were no significant differences in the number of 2-4 cells grades a, b and c embryos and also number of 4-8 cells grades a, b and c, d embryos in groups. conclusion: the concurrent use of ovulation stimulating hormones and gamma rays ameliorates this problem of drastic decrease in number of living embryos due to whole-body irradiation.