Derivation of ES-like cell from neonatal mouse testis cells in autologous sertoli cells co-culture system

Background: spermatogonial stem cell (ssc) is a self-renewing population of male adult stem cell. sscs have a differentiation potential which are similar to embryonic stem cells. these embryonic stem like (es-like) cells can be a potential source for pluripotent cells for stem cell-based therapy.objective: this study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testismaterials and methods: isolated testicular cells were cultured in dmem/f12. characteristics of the isolated cells and obtained es-like cell were immune-cytochemically confirmed by examining the presence of plzf, vimentin, oct4 and nanog protein. expression of the pluripotency and germ-cell specific genes was analyzed by qpcr in derived es-like colony and sscs respectively.results: the experiment results indicated that our method of obtaining pluripotent es-like cells from spermatogonial cells (scs) is simpler than the described methods. es-like cells were immunopositive for pluripotency markers. es-like cell qpcr results indicated significant increase in pluripotency genes expression and significant decrease in germ cell-specific genes expression.conclusion: the results indicated that es-like cell with pluripotency characteristic were generated from freshly isolated spermatogonial cells. the pluripotent stem cells provide a cellular reservoir usable for regenerative medicine instead of embryonic stem cells