The effects of follicular fluid on human X, Y bearingsperm ratio by Fluorescent in Situ Hybridization(FISH)

Background: some evidences showed that the secretion of uterine tube, vagina andfollicular fluid (ff) affects x and y-chromosome populations. sperm selection with xor y chromosome can added to oocyte for gender desired. the isolation of x, y spermhave done and all efforts in this field are done to make culture media similar to in vivocondition. the objective of this study was to find if the ff can influence the ratio of xor y chromosomes, therefore we added human ff to culture media to separate x and ysperms.materials and methods: normal semen sample from 36 healthy men were selected.then the samples were divided into control and experimental groups: control groupsperms have been incubated with conventional culture media (ham's f-10) andexperimental group with conventional culture media + 10% human ff. for spermisolation, swim up technique was used. after 24 hours of incubation, slides smear wereprepared. then, we used the fluorescent in situ hybridization (fish) method toevaluate the effect of follicular fluid on the population ratio of x and y containingsperms.results: although the incubation of sperm in ff and ham's f-10 increased y sperm(59.44 % in control and 61.42% in experimental groups) in comparison with x sperm(40.56% in control and 38.5 in experimental groups) significantly (p<0.05), but the y(or x) bearing sperm did not significantly change in experimental group in comparisonwith y (or x) bearing sperm in control groupconclusion: this study showed that using the swim up method for collecting spermsand adding ff to culture media can improve some sperm parameters, but did not hassignificant effects on population of x and y sperm.