A Rapid and Sensitive Method for Determination of Morphine in Saliva of Opium Addicts

Background: saliva is an alternative biological matrix in drugtesting. it has several advantages in comparison with otherbiological fluids such as blood and urine. c:::jlection of salivais simple, noninvasive and can be controlled under direct observationin drug abusers, or opium addicts.methods: in the present study we report our experience withsaliva collection, liquid-liquid extraction and quali-quantitationof morphine abuse. the gc-ms method was applied to theanalysis and determination of morphine in saliva samples obtainedfrom addicted persons.results: lod of the method was lng/ml and loq 5 ng/ml,with acceptable within-and between-day reproducibility and alinearity (r^2=o.998) over a concentration range from 5 to500ng/m.conclusion: the results of this study indicated that salivamay have potential use for identification of patients suspectedto drug abuse.