assessment of the impact of astragalus floccosus boiss. extract on wound based on phytoestrogenic properties: an in vitro and in vivo study

Background: investigations have shown that estrogen affects wound healing through skin receptors. astragalus floccosus boiss. contains estrogen-like isoflavonoids. this study examined the effects of a. floccosus boiss (a. floccosus) extract on wound healing in vitro and in vivo.methods: for the in vitro test, human fetal foreskin fibroblast 2 (hfff2) cells treated with the root extract of a. floccosus were followed over a 48-hour period. for the in vivo test, rats were categorized into five groups and received daily applications of a cream containing different concentrations of the extract: 5%, 10%, silver sulfadiazine, the ointment base, and no treatment for a duration of 21 days. the wound size and full-thickness wound healing were measured throughout the inspection, and a histological study was conducted as well. the two-way repeated measures anova test was employed, with a significance level of 0.05 (p≤0.05) being used in all cases.results: a. floccosus at 125 ppm significantly improved the closer region and wound healing in hfff2 cells over all periods of time and showed a significant healing effect during 8-12 h (p=0.0234). moreover, an increase in fibrosis (compared to the base (p=0.0005), the 10% extract (p<0.0001), silver (p=0.0005), and free (p<0.0001) groups), epithelization (compared to base (p=0.0006), the 10% extract (p=0.0008), silver (p=0.0007), and free (p=0.0006) groups), and proliferation of fibroblasts (compared to the 10% extract and free groups (p=0.0020 and p=0.0024, respectively) were seen in the 5% extract sample on day 10. conclusion: wound healing processes were observed in rats treated with a 5% a. floccosus extract cream. this finding highlights the potential for future studies on wound healing processes using this extract.