inhibition of ultraviolet b-induced apoptosis in fibroblasts by human umbilical cord blood mesenchymal stem cell conditioned media via the phosphatidylinositol-3-kinase/akt pathway

Background: ultraviolet b (uvb) irradiation induces photoaging and apoptosis in various cell types. inhibition of uvb-induced apoptotic pathways has been explored in different apoptotic cascades. conditioned media from human umbilical cord blood mesenchymal stem cells (cm-hucb-msc) contain important substances for cell regeneration. however, the potential of cm-hucb-msc in preventing uvb-induced apoptosis has not been clearly elucidated. therefore, the current research was conducted to investigate the potential of cm-hucb-msc in inhibiting uvb-induced apoptosis and its role in the antiapoptotic signaling pathway.methods: an experimental in vitro study was conducted at pt. prodia stemcell indonesia, jakarta, indonesia, 2019-2022. initially, hucb-mscs were isolated and cultured to produce cm-hucb-msc. nih3t3 cells were pretreated with/without 50 μm ly294002, treated with/without 10% cm-hucb-msc, and then irradiated with/without uvb. subsequently, the cells were analyzed using sub-g1, immunofluorescence, and immunoblotting assays. one-way analysis of variance (anova) was used for data analysis, followed by tukey’s honest significant difference (hsd) test or the kruskal-wallis test, followed by the dunn-bonferroni test using ibm spss statistics software version 21. statistical significance was determined at p<0.05.results: cm-hucb-msc significantly inhibited uvb-induced apoptosis in nih3t3 cells (p=0.002, dunn-bonferroni test). cm-hucb-msc significantly induced akt phosphorylation at ser 473 in uvb-irradiated nih3t3 cells (p<0.001, tukey’s hsd test). the cm-hucb-msc-induced phosphorylation of akt was significantly inhibited by ly294002 (p<0.001, tukey’s hsd test).conclusion: taken together, it can be concluded that cm-hucb-msc inhibits uvb-induced nih3t3 cell apoptosis via the activation of phosphatidylinositol-3-kinase (pi3k)/akt signaling cascades.