Optimization of the Static Human Osteoblast/Osteoclast Co-culture System

Osteoblasts (obs) and osteoclasts (ocs) are 2 major groups of bone cells. their cell-to-cell interactions are important to ensure the continuity of the bone-remodeling process. therefore, the present study was carried out to optimize an ob/oc co-culture system utilizing the human ob cell line hfob 1.19 and ocs extracted from peripheral blood mononuclear cells (pbmncs). it was a 2- step procedure, involving the optimization of the ob culture and the co-culture of the obs with pbmncs at an optimum ratio. firstly, pre-obs were cultured to 90% confluency and the time required for differentiation was determined. ob differentiation was determined using the van gieson staining to detect the presence of collagen and alizarin red for calcium. secondly, obs and ocs were co-cultured at the ratios of 1 oc: 1 ob, 1 oc: 4 obs, 2 ocs: 1 ob, and 1 oc: 2 obs. tartrate- resistant acid phosphatase (trap) staining was used to detect the differentiation of the ocs. the results showed that collagen was present on day 1, whereas calcium was detected as early as day 3. based on the result of trap staining, 1 oc: 2 obs was taken as the most appropriate ratio. no macrophage colony- stimulating factor and receptor activator of the nuclear factor-kb ligand were added because they were provided by the obs. in conclusion, these optimization processes are vital as they ensure the exact time point and ratio of the ob/oc co-culture in orderto produce a reliable and reproducible co-culture system.