Discrimination and preliminary selection of self-compatible progenies among controlled crosses in almond using Sf specific primer

Self-incompatibility in almond and prunus species is an important trait that prevents self-fertilization. self-incompatibility in almond is controlled gametophytically by the multiallelic s-locus. present study was done in order to identification and preliminary selection of self compatible progenies resulted from controlled crosses in almond using specific primer sff/sfr. some important morphological traits of parental crosses were evaluated using almond descriptor. also, progenies (f1) of five crosses inculding; a (tuono × 101 genotype), b (supernova × 101 genotype), c (genco × shahrood 21), d (tuono × shahrood 12) and e (tuono × shahrood 17) were tested using pcr in order to dna amplification and self-compatibility evaluation. the result of pcr method found that using sff/sfr pair primer, self-compatible progenies showed 449 bp bands, while this band not observed in self-incompatible progenies. in addition, all self- incompatible progenies appeared no s1 allele at any condition. according to chi-square test, ratios of self-compatible to self-incompatible progenies were to mendelian principles. it can be suggested that pcr method of this research has high potential in order to distinguish self-compatible and incompatible genotypes.