evaluation of mir-155 expression in cystic fibrosis patients

Background: cystic fibrosis (cf) is the most fatal genetic disorder among white populations. it is a multisystem disease with various symptoms, which causes many different complications. mirnas are a class of endogenous small noncoding rnas of 1922 nt that regulate mrnas posttranscriptionally. different types of mirnas, ibackground: cystic fibrosis (cf) is the most fatal genetic disorder among white populations. it is a multi-system disease withvarious symptoms, which causes many different complications. mirnas are a class of endogenous small non-coding rnas of 19-22nt that regulatemrnaspost-transcriptionally. different types of mirnas, including mir-155, are expressed in cf lung epithelial cells.the elevated expression level of mir-155 contributes to the pro-inflammatory expression of il-8 in cf lung epithelial cells. therefore,mir-155 may play an important role in the activation of il-8 in cf.objectives: the present study aimed to investigate the relationship between mir-155 expression level and the clinical manifestationsof cf patients.methods: the participants of this cross-sectional study included 30 cf patients (according to sweat test and < f508 in the geneticstudy) with age ranging from 5 to 27 years (m = 17, f = 13) and 30 healthy individuals with age ranging from 8 to 28 (m = 17, f = 13). usingrna purification kit, micrornas, we extracted micrornas from serum samples, and cdna was synthesized via the cdna synthesiskit. then, the levels of mir-155 were measured by real-time pcr, and the expression levels were compared in different groups of cfpatients according to the shwachman-kulczycki scoring system.results: the expression level of mir-155 was elevated in cf patients compared to healthy controls (fold change: 1.41, p value = 0.056).interestingly, the elevated level of mir-155 in severe and moderate patients (according to shwachman-kulczycki score) showed asignificant difference compared to the other patients, where age and sex were not an influential factor.conclusions: serum expression levels of mir-155 are different in cf patients, along with the severity of the disease. by suppressingthe expression of mir-155, more investigations might background: cystic fibrosis (cf) is the most fatal genetic disorder among white populations. it is a multi-system disease with various symptoms, which causes many different complications. mirnas are a class of endogenous small non-coding rnas of 19-22 nt that regulatemrnaspost-transcriptionally. different types of mirnas, including mir-155, are expressed in cf lung epithelial cells. the elevated expression level of mir-155 contributes to the pro-inflammatory expression of il-8 in cf lung epithelial cells. therefore, mir-155 may play an important role in the activation of il-8 in cf. objectives: the present study aimed to investigate the relationship between mir-155 expression level and the clinical manifestations of cf patients. methods: the participants of this cross-sectional study included 30 cf patients (according to sweat test and < f508 in the genetic study) with age ranging from 5 to 27 years (m = 17, f = 13) and 30 healthy individuals with age ranging from 8 to 28 (m = 17, f = 13). using rna purification kit, micrornas, we extracted micrornas from serum samples, and cdna was synthesized via the cdna synthesis kit. then, the levels of mir-155 were measured by real-time pcr, and the expression levels were compared in different groups of cf patients according to the shwachman-kulczycki scoring system. results: the expression level of mir-155 was elevated in cf patients compared to healthy controls (fold change: 1.41, p value = 0.056). interestingly, the elevated level of mir-155 in severe and moderate patients (according to shwachman-kulczycki score) showed a significant difference compared to the other patients, where age and sex were not an influential factor. conclusions: serum expression levels of mir-155 are different in cf patients, along with the severity of the disease. by suppressing the expression of mir-155, more investigations might lead to the development of new treatment strategies for cf. keywords: keywords: cystic fibrosis, mir-155, shwachman-kulczycki score, real-time pcrncluding mir155, are expressed in cf lung epithelial cells. the elevated expression level of mir155 contributes to the proinflammatory expression of il8 in cf lung epithelial cells. therefore, mir155 may play an important role in the activation of il8 in cf. objectives: the present study aimed to investigate the relationship between mir155 expression level and the clinical manifestations of cf patients. methods: the participants of this crosssectional study included 30 cf patients (according to sweat test and