Human Erythrocyte Superoxide Dismutase Encapsulated in Positively Charged Liposomes

Superoxide dismutase (sod) is an important antioxidant that protects many typesof cells from the free radical damage. one of the possible ways for the use of sodis its incorporation in liposomes. the aim of this study was to investigate the effectof cationic phospholipids on the entrapment of human erythrocyte superoxidedismutase (cu/zn sod) in liposomes. also, in the present study, we examined theeffect of this formulation on the permeability of these liposomes for sod at twodifferent temperatures (4 °c and 37 °c). cu/zn sod was purified from humanerythrocytes. several methods, including, precipitation by acetone, chloroform,centrifugation and also ion exchange chromatography on deae-32 were applied.in this study, encapsulated sod in liposomes was prepared using the film hydrationmethod. the results obtained from the prepared human erythrocyte sod showedthat at the end of the last stage, the purification was 21 times the result of the firststage, with a specific activity of 3000 u/mg. the enzyme activity and the retainedenzymatic