Anxiolytic and Antistress Potentials of Ethanol Stem-Bark Extract of Milicia Excelsa (Moraceae) in Mice

Milicia excelsa stem bark is applied in traditional medicine in some african societies, primarily as a tonic to rejuvenate the body after demanding episodes. however, there is a paucity of scientific evidence to support this usage. therefore, this research aimed to evaluate the anxiolytic and anti-stress potentials of ethanol extract of the stem bark of milicia excelsa (esbme) in mice. the central nervous system inhibitory effect of the extract was determined using novelty-induced rearing, grooming, and locomotion behaviors while the anxiolytic effect was investigated by using a hole board and elevated plus maze (epm) test models. the extract’s ability to alleviate the anxiety-like and depressive-like behaviors triggered by acute restraint stress was evaluated with the use of epm and tail suspension test respectively. the esbme significantly (p<0.05) decreased the novelty-induced rearing, grooming, and locomotion behaviors indicating a central nervous system inhibitory effect. at 37.5 mg/kg, esbme significantly (p<0.05) increased the number of head poking in the hole board test designating anxiolytic potential. subsequently, the esbme significantly (p<0.05) increased the percentage of open-arm entries and percentage open-arm duration as well as reducing the anxiety index on elevated plus maze consistent with the antianxiety effect. the extract also significantly (p<0.05) alleviated the anxiety-like and depressive-like behaviors triggered by acute restraint stress suggesting an anti-stress effect. in conclusion, esbme possesses central nervous system inhibitory, anxiolytic, and anti-stress effects thereby providing scientific evidence to the ethnomedicinal claim of the plant as an anti-stress agent.