potency of mangosteen pericarp extract to inhibit 38-kda and ag85 protein secretion by mycobacterium tuberculosis h37rv

Tuberculosis (tb) is an infectious disease caused by mycobacterium tuberculosis. the α-mangostin in mangosteen pericarp extract can inhibit m. tuberculosis growth. this study examined the potency of α-mangostin in mangosteen pericarp extract to inhibit 38-kda and ag85 protein secretion from m. tuberculosis h37rv. the samples used in this study were divided into three independent variable groups (3.125 μg/ml; 6.25 μg/ml; and 12.5 μg/ml α-mangostin in the mangosteen pericarp extract), a positive control group (rifampin), a negative control group (m. tuberculosis h37rv), and the garcia® group (trademark of the mangosteen peel extract capsule). hplc-ms/ms detected that the mangosteen pericarp extract contained 5,984.55 μg/g α-mangostin. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and dot blot analysis were conducted to analyze the profile and specificity of both proteins. this study revealed that 6.25 μg/ml of mangosteen pericarp extract had the greatest potency for inhibiting 38-kda protein secretion from m. tuberculosis h37rv, and 12.5 μg/ml mangosteen pericarp extract had greater potency for inhibiting ag85 protein secretion than did the other doses. thus, α-mangosteen from mangosteen pericarp extract can inhibit ag38 and ag85 secretion.