Simultaneous Estimation of Amlodipine Besylate and Nebivolol Hydrochloride in Pharmaceutical Tablets Formulation by RP-HPLC Using PDA Detector

The present work was undertaken with the aim to develop and validate a rapid and consistent rp-hplc method in which the peaks will be appear with short period of time as per ich guidelines.the separation was achieved on a stainless steel analytical column, eclipse xdb plus c18 column (4.6 x 150 mm; 5 μm) in an isocratic mode. the mobile phase was composed acetonitrile and 0.01 m ammonium acetate (ph adjusted to 4.5 using glacial acetic acid), which were mixed in the ratio of 50: 50. the flow rate was monitored at 1.0 ml/min. the wavelength selected for detection was 265 nm. the retention time found for amlodipine besylate and nebivolol hydrochloride was 2.967 and 3.510 min, respectively. the % recovery was 100.20- 100.86 for amlodipine and 100.20 - 100.78 for nebivolol. the linearity was established in the range of 5-25 μg/ml for amlodipine and 10-50 μg/ml for nebivolol. the slope, intercept, and correlation coefficient were found to be 314.2x, +162.4, and 0.999 for amlodipine besylate and 248x, -305.7, and 0.9998 for nebivolol hydrochloride, respectively. the limits of detection for amlodipine besylate and nebivolol hydrochloride obtained by the proposed method was 0.07 and 0.20 μg/ml and the limits of quantification for amlodipine besylate and nebivolol hydrochloride obtained by the proposed method was 0.23 and 0.61 μg/ml, respectively. the method was found to be suitable for the quality control test of amlodipine besylate and nebivolol hydrochloride simultaneously in a bulk drugs as well as in a formulations.