High Performance Liquid Chromatography Determination of Sertraline in Human

An accurate and sensitive reversed-phase high-performance liquid chromatographic method for determination of sertraline in human serum is described using4-chloro-7-nitrobenzofurazan as pre-column derivatization agent. the drug and aninternal standard (azithrimycin) were extracted from serum using a mixture ofdiethyl ether-chloroform and subjected to the pre-column derivatization with thereagent. analysis of the resulted derivatives was performed on a lichrosorb cn(250×4.0 mm) column using a mobile phase composed of methanol and sodiumphosphate buffer (0.05 m; ph 3.7) containing 2 ml/lit triethylamine (63:37 v/v).detector response was monitored at excitation and emission wavelengths of 470 and 537 nm, respectively. the calibration curve was linear over the concentration rangeof 2 to 640 ng/ml. the lower limits of detection and quantification were 0.5 and 2 ng/ml, respectively. the validation of the analysis was carried out in terms ofspecificity, sensitivity, linearity, precision, accuracy and stability. the validated methodwas shown to be accurate, with intra-day and inter-day accuracy from 0.3 to 4.2% and precise, with intra-day and inter-day precision from 2.4 to 15.5%. the drug isdetected at concentrations as low as 2 ng/ml in a 0.5 ml serum sample and thedescribed method can be easily applied in human single-dose pharmacokinetic studies of sertraline.