Development and Application of a Validated Liquid Chromatography-Mass Spectrometry Method for the Determination of Dexchlorpheniramine Maleate in Human Plasma

A convenient liquid chromatographic-single quadrupole mass spectrometric(lc-ms) method was developed and validated for dexchlorpheniramine maleate (innname: chlorphenamine) determination in human plasma. the need for just a singleliquid-liquid extraction with ethyl acetate and being highly sensitive were theadvantages of this method. the linearity was also excellent over the range of 1 to150 ng.ml-1 of dexchlorpheniramine maleate concentration. the method wasstatistically validated for its selectivity, linearity, precision and robustness. this methodwas successfully applied to the analysis of chlorpheniramine maleate in clinicalstudies.