Synthesis and Characterization of Biodegradable Hemostat Gelatin Sponge for Surgery Application

Production and characterization of soft cross-linked gelatin sponge by usingglutaraldehyde for blood hemostasis applic ation, is the goal of this study.biodegradable hydrogels were prepared through crosslinking of gelatin withglutaraldehyde followed by freeze drying. the effects of gelatin concentration, amountof crosslink agent and freeze drying temperature on mechanical properties andelasticity, stability and degradation rate , swelling and water absorption ability,hemostatic effect and cytotoxicity were investigated. as the freezing temperaturewas lowered, the density of freeze-dried sponges increased. density of samples isstrongly dependent on the freezing temperature before freeze-drying . the additionof gta as cross linker agent changed density very slightly, but no direct relation shipbetween the amount of cross linker and the density was observed. gelatin spongesprepared by freeze-drying after freezing at -] 0 and -2s °c had large pores . networkstructure of heterogeneous pore s. the rate of weight loss decreased with increasingdegree of crosslinking of the samples, and the higher degree of cross-linking in gelatinsponges causes more resistance to degradation in pbs solution. gelatin concentrationincrease had very sharp effect on raising the compression module, and freezingtemperature changed inner structure of sponges and can affect mechanical behavior.