Effect of Alkaline-Acid Treatment on the Biomimetic Formation of Calcium Phosphate Layers on Titanium Substrates: An in Vitro Study

The aim of this study was to investigate the effect of alkaline and heat treatmentand acid etching of titanium substrates on the formation of calcium phosphatelayer and bioactivity in simulated body fluid (sbp) solution. in this research, at firstthe titanium surfaces were treated with 5n naoh, 15% hn03-lo%hp-75% h20and 5n naoh followed by heating at 600 °c for lh. the samples were immersedin the sbp for 28 days to investigate the influence of surface treatments of titaniumon in vitro bioactivity. the modified substrates were characterized using scanningelectron microscopy, x-ray diffraction and profilometry of samples were investigatedby light interferometry, the in vitro assessment was carried out through theimmersion of samples in sbf. according to the results obtained in this work thedeposit of calcium phosphate in alkali and alkali/heat treated samples were morestable than acid etched samples. porous network structure containing the sodiumtitanate and the titanium oxide was characterized on the surface after the titaniumwas subjected to naoh aqueous solution. results have illustrated the fact thatchemical surface treatment improves in vitro behavior of titanium.