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   comparison of three pcrbased methods for simplicity and cost effectiveness identification of cutaneous leishmaniasis due to leishmania tropica  
   
نویسنده mohammadi mohammad ali ,bamorovat mehdi ,fasihi harandi majid ,karimi tayyebeh ,sharifi iraj ,aflatoonian mohammad reza
منبع iranian journal of parasitology - 2017 - دوره : 12 - شماره : 2 - صفحه:215 -223
چکیده    Background: to compare three molecular methods, pcr-rflp for internal transcribed spacer, pcr sequencing and high resolution melting analysis shown reliable sensitivity and specificity for detecting leishmania tropica as a model for cutaneous leishmaniasis (cl) as the perspective overview for scientific and economic approaches. methods: this study was carried out between 2015 and 2016 in leishmaniasis research center in kerman university of medical sciences, kerman, iran. the positives smears (n=50) were obtained from patients referred from the health clinics in a major anthroponotic cl (acl) focus, southeastern iran. only smear preparations with the same grade were selected according to the method described by the who for future pcr assays. results: all three molecular methods had capability to identify positive samples at species level with the same specificity and sensitivity. however, these techniques were different in simplicity, consuming time, and cost effectiveness. although additional enzymatic process in pcr-rflp provided good resolution to find leishmania species but this would cause time and cost increases. conclusion: hrm (high resolution melting) is a relatively new technique that allows direct characterization of pcr amplicons in a closed system with more simplicity, cost effectiveness and time-consuming compared with other pcr-based assays for epidemiological or clinical identification purposes.
کلیدواژه molecular identification ,cutaneous leishmaniasis ,pcr ,iran
آدرس kerman university of medical sciences, research center for hydatid disease in iran, ایران, kerman university of medical sciences, leishmaniasis research center, ایران, kerman university of medical sciences, research center for hydatid disease in iran, ایران, kerman university of medical sciences, school of medicine, dept of medical parasitology, ایران, kerman university of medical sciences, leishmaniasis research center, ایران, kerman university of medical sciences, research center for tropical and infectious diseases, ایران
پست الکترونیکی aa@yahoo.com
 
     
   
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