Cloning and Sequencing of Leishmania major Thiol-Specific- Antioxidant Antigen (TSA) Gene

Background: leishmaniasis is caused by parasitic protozoa of the genus leishmania which, in the infected host are obligateintracellular parasite. tsa is the immuno-dominant antigen of leishmania major which is considered as the mostpromising molecule for a recombinant or dna vaccine against leishmaniasis.methods: genomic dna of tsa protein was extracted and amplificated as a template. then the pcr product was clonedinto ptz57r/t vector. finally, the recombinant plasmid was extracted from transformed escherichia coli (tg1 strain) andsequenced.results: mrho/ir/75/er (an iranian strain) of l. major and tsa gene (accession number lmjf15.1080) were used. sequenceanalysis of cloned tsa gene into ptz57r/t vector showed high homology of 90% with lmjf15.1080 (tsa gene)and strain lv39 (accession no. af069386) and strain friedlin (accession no.af044679).conclusion: we cloned tsa gene of l. major successfully. recombinant plasmid was confirmed. it is ready to expressrecombinant protein for further studies.