Evaluation of Dot-ELISA Method Using Excretory-Secretary Antigens of Fasciola hepatica in Laboratory Diagnosis of Human Fasciolosis

Fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. in this study, dot-elisa, one of the simplest and the most sensitive tests in this regard, wasevaluated using excretory-secretory antigens of fasciola hepatica to diagnose human fasciolosis three groups consisting of patients infected with fasciolosis (n= 95), patients with other parasitic diseases (n= 37)and healthy individuals (n= 40), were implicated in the test. all collected sera were tested by dot-elisa using excretory-secretory antigens. optimal criteria were detected as 1.5 ug of antigen per dot, serum dilution of 1:320, and anti human igg conjugate dilution of 1:500. the sensitivity, specificity, positive and negative predictivevalues were 96.8%, 96.1%, 96.8% and 96.1%, respectively. in conclusion, dot-elisa using excretory- secretory antigens could be regarded as a cheap, rapid, antigen and serum conservative diagnosticmethod in diagnosing fasciolosis.