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genetic identification of orientobilharzia turkestanicum from sheep isolates in iran.
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نویسنده
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tabaripour reza ,youssefi mohammad reza ,tabaripour rabeeh
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منبع
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iranian journal of parasitology - 2015 - دوره : 10 - شماره : 1 - صفحه:62 -8
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چکیده
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background: adult worms of orientobilharzia turkestanicum live in the portal veins, or intestinal veins of cattle, sheep, goat and many other mammals caus-ing orientobilharziasis. orientobilharziasis causes significant economic losses to livestock industry of iran. however, there is limited information about genotypes of o. turkestanicum in iran. methods:in this study, 30 isolates of o. turkestanicum obtained from sheep were characterized by sequencing mitochondrial cytochrome c oxidase subunit 1 (cox1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1) gene. the mitochondrial cox1 and nad1 dna were amplified by poly-merase chain reaction (pcr) and then sequenced and compared with o. turke-stanicum and that of other members of the schistosomatidae available in gen- banktm. results:phylogenetic relationships between them were re-constructed using the maximum parsimony method. phylogenetic analyses done in present study placed o. turkestanicum within the schistosoma genus, and indicates that o. turkestanicum was phylogenetically closer to the african schistosome group than to the asian schistosome group. conclusion:comparison of nad1 and cox1 sequences of o. turkestanicum ob-tained in this study with corresponding sequences available in genbanktm re-vealed some sequence variations and provided evidence for presence of mi-crovarients in iran. |
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کلیدواژه
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iran ,orientobilharzia turkestanicum ,sheep
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آدرس
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dept. of cellular and molecular biology, islamic azad university babol-branch, iran., Iran, Islamic Republic of, dept. of veterinary parasitology, islamic azad university babol-branch, iran., Iran, Islamic Republic of, young researchers club, islamic azad university, babol branch, babol, iran., Iran, Islamic Republic of
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پست الکترونیکی
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youssefi929@hotmail.com
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Authors
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