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efficiency of nested-pcr in detecting asymptomatic cases toward malaria elimination program in an endemic area of iran.
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نویسنده
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turki habibollah ,raeisi ahmad ,malekzadeh kianoosh ,ghanbarnejad amin ,zoghi samaneh ,yeryan masoud ,abedi nejad masoumeh ,mohseni fatemeh ,shekari mohammad
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منبع
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iranian journal of parasitology - 2015 - دوره : 10 - شماره : 1 - صفحه:39 -45
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چکیده
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background: the aim of this study was to detect low parasite and asymptomatic malaria infections by means of three malaria diagnostic tests, in a low transmission region of minab district, hormozgan province, southern iran. methods:blood samples of 200 healthy volunteers from bagh-e-malek area were evaluated using microscopic, rapid diagnostic tests (rdt) and nested-pcr to in-spect malaria parasite. results:the results showed no plasmodium parasite in subjects by means of mi-croscopy and rdt. however, 3 p. vivax positive samples (1.5%) were discovered by nested-pcr while microscopy and rdt missed the cases. conclusion:microscopy as the gold standard method and rdt correctly identi-fied 98.5% of cases, and molecular analysis is sensitive and reliable, especially in the detection of asymptomatic infections for active case surveillance. regarding the existence of asymptomatic malaria in endemic area of hormozgan, iran, nest-ed-pcr could be considered as a sensitive tool to interrupt malaria transmission in the country, beside the microscopic and rdt methods. |
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کلیدواژه
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asymptomatic malaria ,malaria elimination ,nested-pcr
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آدرس
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hormozgan university of medical sciences, ایران, ministry of health and medical education, national programme manager for malaria control, ایران, hormozgan university of medical sciences, ایران, hormozgan university of medical sciences, social determinants in health promotion research center, ایران, tehran university of medical sciences tums, ایران, hormozgan university of medical sciences, ایران, hormozgan university of medical sciences, ایران, hormozgan university of medical sciences, ایران, hormozgan university of medical sciences, ایران
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پست الکترونیکی
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mshekari92@yahoo.com
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Authors
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