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Comparison of Scolicidal Effects of Amphotricin B, Silver Nanoparticles,_And Foeniculum Vulgare Mill on Hydatid Cysts Protoscoleces.
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نویسنده
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Lashkarizadeh Mohammad Reza ,Asgaripour Keivan ,Saedi Dezaki Ebrahim ,Fasihi Harandi Majid
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منبع
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Iranian Journal Of Parasitology - 2015 - دوره : 10 - شماره : 2 - صفحه:206 -212
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چکیده
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background:haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. the present study was carried out to differentiate haemonchus species from its main hosts in iran, including sheep, goat and camel.
methods:the identification took place based on the morphometrics of the spic-ules and molecular characters. two hundred seventy adult male nematodes were collected from the abomasums of different ruminants (90 samples from each ani-mal) at the slaughterhouses from different localities in iran. samples were morpho-logically identified according to the spicules’ morphometric measurements. in the section on molecular study, 10 samples of each haemonchus isolates were genetically examined. a simple pcr-restriction fragment length polymorphism (pcr-rflp) assay of the second internal transcribed spacer of ribosomal dna (its2-rdna) were described to confirm the pcr results.
results:pcr-rflp profile obtained from the restriction enzyme hpa1 in h. con-tortus and h. longistipes indicated 1 (278 bp) and 2 (113 and 135 bp) different frag-ments, respectively. the morphological parameters clearly distinguish h. contortus from h. longistipes. moreover, regarding the its2-rdna, sequences of 295 bp and 314 bp were obtained from h. contortus and h. longistipes, respectively.
conclusion:high similarity of cloned pvmsp-142 kda gene in comparison to reference sequence and other sequences could be beneficial as a remarkable mo-lecular marker for serological diagnostic. |
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کلیدواژه
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Ag-Nps ,Cystic Echinococcosis ,Fennel ,Fungizone ,Hypertonic Saline
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آدرس
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Dept. Of Surgery And The Research Center For Tropical And Infectious Diseases, Kerman University Of Medical Sciences, Kerman, Iran., Iran, Islamic Republic Of, Dept. Of Surgery And The Research Center For Tropical And Infectious Diseases, Kerman University Of Medical Sciences, Kerman, Iran., Iran, Islamic Republic Of, Kerman University Of Medical Sciences, Iran, Islamic Republic Of, Kerman University Of Medical Sciences, Iran, Islamic Republic Of
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پست الکترونیکی
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majid.fasihi@gmail.com
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Authors
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