Cloning and Sequence Analysis of Recombinant Plasmodium Vivax Merozoite Surface Protein 1 (Pvmsp-142 Kda) in Ptz57r/T Vector.

background:haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. the present study was carried out to differentiate haemonchus species from its main hosts in iran, including sheep, goat and camel.
 methods:the identification took place based on the morphometrics of the spic-ules and molecular characters. two hundred seventy adult male nematodes were collected from the abomasums of different ruminants (90 samples from each ani-mal) at the slaughterhouses from different localities in iran. samples were morpho-logically identified according to the spicules’ morphometric measurements. in the section on molecular study, 10 samples of each haemonchus isolates were genetically examined. a simple pcr-restriction fragment length polymorphism (pcr-rflp) assay of the second internal transcribed spacer of ribosomal dna (its2-rdna) were described to confirm the pcr results.
results:pcr-rflp profile obtained from the restriction enzyme hpa1 in h. con-tortus and h. longistipes indicated 1 (278 bp) and 2 (113 and 135 bp) different frag-ments, respectively. the morphological parameters clearly distinguish h. contortus from h. longistipes. moreover, regarding the its2-rdna, sequences of 295 bp and 314 bp were obtained from h. contortus and h. longistipes, respectively.
conclusion:high similarity of cloned pvmsp-142 kda gene in comparison to reference sequence and other sequences could be beneficial as a remarkable mo-lecular marker for serological diagnostic.