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phylogenetic study of haemonchus species from iran based on morpho-molecular characterization.
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نویسنده
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meshgi behnam ,jalousian fatemeh ,masih zahra
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منبع
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iranian journal of parasitology - 2015 - دوره : 10 - شماره : 2 - صفحه:189 -196
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چکیده
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background:haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. the present study was carried out to differentiate haemonchus species from its main hosts in iran, including sheep, goat and camel.
methods:the identification took place based on the morphometrics of the spic-ules and molecular characters. two hundred seventy adult male nematodes were collected from the abomasums of different ruminants (90 samples from each ani-mal) at the slaughterhouses from different localities in iran. samples were morpho-logically identified according to the spicules’ morphometric measurements. in the section on molecular study, 10 samples of each haemonchus isolates were genetically examined. a simple pcr-restriction fragment length polymorphism (pcr-rflp) assay of the second internal transcribed spacer of ribosomal dna (its2-rdna) were described to confirm the pcr results.
results:pcr-rflp profile obtained from the restriction enzyme hpa1 in h. con-tortus and h. longistipes indicated 1 (278 bp) and 2 (113 and 135 bp) different frag-ments, respectively. the morphological parameters clearly distinguish h. contortus from h. longistipes. moreover, regarding the its2-rdna, sequences of 295 bp and 314 bp were obtained from h. contortus and h. longistipes, respectively.
conclusion:the genotypic results are in agreement with the phenotypic findings of both species.
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کلیدواژه
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haemonchus species ,its2 ,morphology ,pcr-rflp
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آدرس
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university of tehran, ایران, university of tehran, ایران, university of tehran, ایران
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پست الکترونیکی
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bmeshgi@ut.ac.ir
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Authors
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