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comparative proteomic profiling of leishmania tropica: investiga tion of a case infected with simultaneous cutaneous and viscero tropic leishmaniasis by 2-dimentional electrophoresis and mass spectrometry
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نویسنده
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hajjaran homa ,mousavi parisa ,burchmore richard ,mohebali mehdi ,mohammadi bazargani mitra ,hosseini salekdeh ghasem ,kazemi-rad elham ,khoramizadeh mohammad reza
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منبع
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iranian journal of parasitology - 2015 - دوره : 10 - شماره : 3 - صفحه:366 -380
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چکیده
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background: viscerotropic leishmaniasis caused by leishmania tropica poses a significant problem in the diagnosis and treatment management. since differential gene expression is more important in outcome of the infection, we employed proteomic approach to identify potential proteins involved in visceralization of l. tropica. methods: the proteomes profiling of l. tropica isolated from cutaneous and visceral tissues of one host were compared by 2-de/ms proteomics study. moreover, the transcript level of some identified proteins was confirmed using real-time rt-pcr. results: of the 700 protein spots that were detected reproducibly on each gel, 135 were found to be differentially expressed (p≤ 0.05). most of responsive proteins in visceral isolate changed in less abundant compared to cutaneous isolate. among differentially expressed proteins, 56 proteins were confidently identified and classified according to the biological process. the largest groups consist of proteins involved in carbohydrate metabolism and protein synthesis. most of the identified proteins, which implicated in energy metabolism, cell signaling and virulence were down-regulated, whereas some proteins that have a role in protein folding, antioxidant defense and proteolysis were up-regulated in visceral form. moreover, the transcript level of some identified proteins such as co-chaperon was confirmed using real-time rt-pcr. conclusion: l. tropica probably uses different mechanisms for survival and multiplication in viscera to establish viscerotropic leishmaniasis. the current study provides some clues into the mechanisms underlying the dissemination of l. tropica.
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کلیدواژه
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l. tropica ,viscerotropic leishmania-sis ,proteome ,2-de ,lc mass spectrom-etry
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آدرس
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tehran university of medical sciences tums, School of Public Health, Department of Parasitology, ایران, tehran university of medical sciences tums, school of public health, dept of medical parasitology and mycology, ایران, university of glasgow, college of medical, veterinary and life science, institute of infection, immunity and inflammation, United Kingdom, tehran university of medical sciences tums, ایران, agriculture institute, iranian research organization for science and technology, ایران, agricultural biotechnology research institute of iran, ایران. Royan Institute for Stem Cell Biology and Technology, ایران, pasteur institute of iran, dept of medical parasitology, ایران, tehran university of medical sciences tums, ایران
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پست الکترونیکی
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hajaranh@tums.ac.ir
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Authors
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