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double-blind randomized efficacy field trial of alum precipi tated autoclaved leishmania major (alum-alm) vaccine mixed with bcg plus imiquimod vs. placebo control group
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نویسنده
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barati mohammad ,mohebali mehdi ,alimoham madian mohammad hossein ,khmesipour ali ,keshavarz hossein ,akhoundi behnaz ,zarei zabihollah
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منبع
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iranian journal of parasitology - 2015 - دوره : 10 - شماره : 3 - صفحه:351 -359
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چکیده
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background: canine visceral leishmaniasis (cvl) is not only an emerging veterinary concern but also a public health threat in endemic areas. the aim of this study was to assess the efficacy, immunogenicity and safety of two doses of aluminum hydroxide (alum) precipitated leishmania major (alum-alm) mixed with bcg plus imiquimod against cvl. methods: a total of 560 ownership dogs were serologically tested and 234 healthy dogs with no clinical signs of cvl, no anti-leishmania antibodies and negative leishmanin skin test were selected and double-blind randomly injected intradermally either with 0.1 ml alum-alm (200µg protein) mixed with bcg (2 × 106 cfus) plus imiquimod (121 dogs) or with 0.1 ml of normal saline (113 dogs). results: the follow-up examinations showed that there was no side effect associated with the vaccination except one case. strong skin test conversion were seen in vaccinated group (30.3%) compared to the control group (6.6%) at 22-24 weeks after the booster injection (p<0.001). the seroconversion was 16.3% (18/110) in vaccinated group and 26.4% (28/106) in control group after two transmission cycles but the difference was not significant (p=0.095). the efficacy rate based on seroconversion was 40.4 %. conclusion: two injections of alum-alm mixed with bcg and imiquimod is safe, although decreases the seroconversion rate of cvl, but the overall efficacy was low.
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کلیدواژه
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alum ,leishmania major ,vaccine ,canine visceral leish-maniasis ,bcg ,imiquimod ,iran
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آدرس
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aja university of medical sciences, ایران. tehran university of medical sciences tums, ایران, tehran university of medical sciences tums, ایران, pasteur institute of iran, dept of immunology, ایران, tehran university of medical sciences tums, ایران, tehran university of medical sciences tums, ایران, tehran university of medical sciences tums, ایران, tehran university of medical sciences tums, school of public health, meshkin-shahr research station, ایران
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پست الکترونیکی
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mohebali@tums.ac.ir
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Authors
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