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Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran
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نویسنده
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Rahdar M ,Niyyati M ,Salehi M ,Feghhi M ,Makvandi M ,Pourmehdi M ,Farnia S
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منبع
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iranian journal of parasitology - 2012 - دوره : 7 - شماره : 4 - صفحه:22 -26
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چکیده
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Background: acanthamoeba spp. are free-living amoebae commonly found in the environmental sources such as water, soil, and air. this ubiquitous amoeba is the causative agent of amoebic keratitis (ak). the objective of the present study was to investigate the presence of acanthamoeba spp. in water and soil sources in ahvaz city, khuzestan province, southern iran. methods: in general, 110 samples of water and soil were taken from different localities of ahvaz including agricultural canals, rivers, and swimming pools. filtration and cultivation were carried out on non-nutrient agar medium (nna). axenic cultivation was performed for all of positive isolates. pcr analysis was conducted on positive samples. sequencing was done for 15 pcr products. genotypes were identified by blast search and homology analysis. result: acanthamoeba spp. was found in 43 (71.6%) of samples of water and 13 (26%) soil samples. genotyping of 15 samples proved that acanthamoeba belonged to t4 (86.6%), t2 (6.6%), and t5(6.6%) genotypes. conclusion: tyi-s-33 medium could be better than pyg medium for acanthamoeba axenic culture.
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کلیدواژه
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Acanthamoeba spp. ,Water ,Soil ,TYI-S-33 ,Iran
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آدرس
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ahvaz jundishapur university of medical sciences, Cellular and Molecular Research Center, Tropical and Infectious Diseases Research Center, School of Medicine, Department of Parasitology, ایران, shahid beheshti university of medical sciences, School of Medicine, Department of Parasitology and Mycology, ایران, ahvaz jundishapur university of medical sciences, Cellular and Molecular Research Center, Tropical and Infectious Diseases Research Center,School of Medicine, Department of Parasitology, ایران, ahvaz jundishapur university of medical sciences, Imam Khomeini Hospital, Department of Ophthalmology, ایران, ahvaz jundishapur university of medical sciences, School of Medicine, Department of Virology, ایران, shahid chamran university of ahvaz, School of Veterinary, Department of Epidemiology and Statistical, ایران, tehran university of medical sciences tums, School of Public Health, Department of Parasitology and Mycology, ایران
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Authors
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