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Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran
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نویسنده
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Habibi GR ,Imani AR ,Gholami MR ,Hablolvarid MH ,Behroozikhah AM ,Lotfi M ,Kamalzade M ,Najjar E ,Esmaeil-Nia K ,Bozorgi S
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منبع
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iranian journal of parasitology - 2012 - دوره : 7 - شماره : 3 - صفحه:64 -72
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چکیده
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Background: the aim of this study was to apply the nested-pcr and bioassay methods in detection and genotyping of toxoplasma gondii infection in provided sheep aborted fetus samples from qazvin province of iran. methods: eighteen sheep aborted fetal samples were studied by nested-pcr-rflp, histopathological observation and microbiological assay. bioassay in mice was carried out by inoculating the brainsamples intraperitoneally. results: the results demonstrated the frequency of 66% infected sheep aborted fetal samples with t. gondii type one. although we could not isolate any parasite from inoculated mice even after three passages, but it was confirmed histopathologically formation of cyst like bodies in prepared mice brainsections. conclusion: the results of the performed nested-pcr and formation of brain cyst in inoculated mice exhibited that t. gondii type one infection might be considered as one of the major causative agents for abortion in ewes.
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کلیدواژه
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Toxoplasma gondii ,Sheep abortion ,Fetus ,PCR ,Bioassay ,Genotyping
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آدرس
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Razi Vaccine and Serum Research Institute, Parasite Vaccine Research and Production Department, ایران, islamic azad university, Veterinary College, ایران, Razi Vaccine and Serum Research Institute, Pathology Department,, ایران, Razi Vaccine and Serum Research Institute, Pathology Department, ایران, Razi Vaccine and Serum Research Institute, Brucellosis Vaccine Research and Production Department, ایران, Razi Vaccine and Serum Research Institute, Quality Control of Biological Products group, ایران, Razi Vaccine and Serum Research Institute, Quality Control of Biological Products group, ایران, Veterinary Organization of Qazvin Province, ایران, Razi Vaccine and Serum Research Institute, Parasite Vaccine Research and Production Department, ایران, Razi Vaccine and Serum Research Institute, Parasite Vaccine Research and Production Department, ایران
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Authors
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