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   subtyping of cryptosporidium parvum obtained from humans and calves in van, turkey  
   
نویسنده ekici abdurrahman ,unlu ahmet hakan ,aydemir selahattin ,barlik fethi ,hasan
منبع iranian journal of parasitology - 2022 - دوره : 17 - شماره : 3 - صفحه:366 -374
چکیده    Background: we aimed to investigate the prevalence of cryptosporidium species detected in humans and calves in the van region of turkey.methods: a total of 150 patients, comprising 60 who were immunosuppressed, 50 who were immunosuppressed and had diarrhea, and 40 who had only diarrhea, were enrolled in this study in the department of medical parasitology, van yuzuncu yıl university faculty of medicine, turkey. stool samples were taken from the rectums of a total of 50 calves that had 30 diarrhea and 20 that did not have diarrhea, from the stables and farms of 10 central villages of van, turkey. all samples were analyzed using modified acid-fast staining, immunochromatographic test, and pcr. cryptosporidium positive samples were also subtyped.results: only c. parvum subtypes were detected in all positive samples. c. parvum was detected in 30 (20%) of the 150 human stool samples, while it was detected in 5 (10%) of the 50 samples from the calves. the gp60 gene region was amplified and sent for sequence analysis to identify the c. parvum subtypes.conclusion: as a result, c. parvum is found to be an active species that caused cryptosporidiosis is in the van region. iida24g1 subtype of c. parvum were found in both human and calf. therefore, due to the zoonotic feature of the c. parvum iida24g1 subtype, it has been shown that the calves in the region are a significant risk for humans.
کلیدواژه cryptosporidium parvum ,subtyping ,gp60 subtype ,immunochromatographic test ,modified acid-fast staining
آدرس van yuzuncu yil university, faculty of medicine, department of parasitology, turkey, van yuzuncu yil university, vocational school of gevas, division of veterinary, turkey, van yuzuncu yil university, faculty of medicine, department of parasitology, turkey, van yuzuncu yil university, van health services vocational school, turkey, van yuzuncu yil university, faculty of medicine, department of parasitology, turkey
پست الکترونیکی hasanyilmazvan@hotmail.com
 
     
   
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