Trypanothione Reductase Gene Mutations in Meglumine Antimoniate Resistant Isolates from Cutaneous Leishmaniasis Patients Using Molecular Dynamics Method

Background: in this study, mutations in the tripanothion reductase of leishmaniatropica isolated from iran was investigated using sequencing and simulation of theenzyme by the molecular dynamic method.methods: fifteen susceptible and 15 clinical resistant l. tropica specimens werecollected from skin lesions from different regions of iran in 2017. after dna extraction,trypanothione reductase (tryr or tpr), gene fragment was amplifiedusing pcr and sequencing methods. in the case of structural mutations, the componentswere simulated by molecular dynamics using the gromacs software.results: some structural mutations were observed in 9 amino acids surroundingthe active site of the tryr gene of l. tropica with three-dimensional trypanothionereductase alteration.conclusion: change in the active site of tryr of l. tropica, could probably contributeto the development of resistant l. tropica to glucantime. because of the likelyoccurrence of mutations in glucantime as well as the ease of development of l.tropica resistant populations, more samples are needed to demonstrate the relationshipbetween mutations in this enzyme and clinical resistance to glucantime. onthe other hand, it is recommended that enzymatic studies be performed to confirmthe role of mutation in the function and expression of trypanothione reductase inglucantime resistant and susceptible populations