Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis

Considering that elisa method presently is the test of choice for diagnosis offasciolosis, the present study was undertaken to evaluate the maximum validity of coated plates atdifferent temperatures and different times during one year of evaluation.methods: serum samples of patients infected with fasciolosis (n=10), hydatidosis (n=5),toxocariasis (n=5), and negative control sera (n=5) were examined. two series of plates wereconsidered. the first series were coated with fasciola homogenate ag 12 ug/ml, and after somesteps were blocked with gelatin and preserved at different temperatures as -80 °c , -20 °c, -4 °cand +4°c. the 2nd series were treated under the same criteria but were not blocked with gelatin.each series were examined by elisa test from 1st month to 12th month. sera with 1:125 dilution,and peroxidase-conjugated goat anti-human igg diluted 1:10000 were considered optimum.results: to ease reporting the results and due to many similarities only results related to 1st, 6thand 12th months were analyzed and sensitivity, specificity plus cut-off were determined for eachseries separately.conclusion: preserving the coated plates, while unblocked at -80°c for 6-8 months is pertinentand functional and in that case, we can be sure the best out put would be applicable.