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Mini-Exon Genotyping of Leishmania Species in Khuzestan Province, Southwest Iran
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نویسنده
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Saki J ,Meamar AR ,Oormazdi H ,Akhlaghi L ,Maraghi S ,Mohebali M ,Khademvatan S ,Razmjou E
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منبع
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iranian journal of parasitology - 2010 - دوره : 5 - شماره : 1 - صفحه:25 -34
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چکیده
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Leishmaniasis is a protozoan disease cause by leishmania genus. anthroponotic andzoonotic cutaneous leishmaniasis are endemic in iran. the aim of this study was to identify the causativeagent of cutaneous leishmaniasis by mini-exon gene in five regions of khuzestan province, southwest ofiran.methods: from 2007 to 2008 in this cross-sectional study, cutaneous samples were collected frompatients referred to health centers and hospitals of the khuzestan province for cutaneous leishmaniasisdiagnosis and cultured in novy-macneal-nicolle (nnn) and rpmi 1640. the propagated promastigoteswere harvested and leishmania species of cutaneous leishmaniasis were identified by rflp and dnasequencing of the pcr generated fragments.results: l. major and l. tropica were the causative agents of cutaneous leishmaniasis by predominantlyof l. major species. the alignment of the mini-exon sequencing isolates with reported sequencing of l.major and l. tropica revealed 92%-99% identity.conclusion: our study showed that mini-exon pcr-rflp was useful method to identify the causativespecies of cutaneous leishmaniasis.
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کلیدواژه
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Cutaneous leishmaniasis ,Mmini-exon ,RFLP ,Sequencing ,Iran
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آدرس
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iran university of medical sciences, Faculty of Medicine, Department of Medical Parasitology and Mycology, ایران. ahvaz jundishapur university of medical sciences, Faculty of Medicine, Department of Medical Parasitology and Mycology, ایران, iran university of medical sciences, Faculty of Medicine, Department of Medical Parasitology and Mycology, ایران, iran university of medical sciences, Faculty of Medicine, Department of Medical Parasitology and Mycology, ایران, iran university of medical sciences, Faculty of Medicine, Department of Medical Parasitology and Mycology, ایران, ahvaz jundishapur university of medical sciences, Faculty of Medicine, Department of Medical Parasitology and Mycology, ایران, tehran university of medical sciences tums, School of Public Health, Department of Medical Parasitology and Mycology, ایران, ahvaz jundishapur university of medical sciences, Faculty of Medicine, Department of Medical Parasitology and Mycology, ایران, iran university of medical sciences, Faculty of Medicine, Department of Medical Parasitology and Mycology, ایران
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پست الکترونیکی
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razmjou@iums.ac.ir
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Authors
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