Carbohydrate Detection and Lectin Isolation from Tegumental Tissue of Fasciola hepatica

Fascioliasis is a chronic hepatic disease and may be resulted from mechanical/molecular parasite adhesion to host liver tissue. the aim of this study was to detect surfacecarbohydrate and lectin, carbohydrate-binding protein isolation that might be responsible ofthis molecular binding.methods: the present experimental work was conducted in the department of medicalparasitology and mycology, school of public health, tehran university of medical sciences,tehran, iran. fasciola hepatica parasites were collected from abattoir (saman, tehran, iran)and surface mannose-carbohydrate was detected by fluorescein isothiocyanate (fitc) conjugatedlectin (lentil). lectin of tegumental tissue from f. hepatica was isolated by affinitychromatography and detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis(sds-page).results: mannose carbohydrate was observed on the surface of tegumental tissue from parasiteunder fluorescence microscope. carbohydrate-binding protein or lectin with mw of 50kda also was isolated from homogenized tegument of helminth.conclusion: these results are important for understanding of molecular pathogenesis of f.hepatica at the chronic phase of fascioliasis