anti-toxocara seropositivity in the patients with eye diseases in ophthalmology clinics in tehran, iran

Background: eye diseases, including, uveitis, inflammation of the retina, and choroid are caused by different agents in humans and has a variety of anterior medial and posterior types. the agent of eye diseases is very different from simple bacterial to acute viral, fungal and parasitic infection. there is limited information regarding the type of eye diseases and toxocara canis. methods: blood samples of 359 individuals (339 patients including endophthalmitis, uveitis, dcr, glaucoma, and cataract 20 individuals as control group) together with their information were collected from ophthalmology hospitals in tehran, iran from feb 2013 to jan 2015. the patient's serum was evaluated for the presence of anti-toxocara antibodies by elisa kits and blood smears for high eosinophilia. the positive samples were confirmed by western blot analysis for t. canis infection. results: overall, 339 patients sera with eye diseases and control group were tested for anti-t. canis antibodies. nineteen (5.6%) patients had anti-t. canis igg that 14 (6.1%) were male and 5 (4.5%) were female, and all the patients had negative eosinophilia as well. the results of western blot analysis for 19 positive patients indicated that 15 were infected by t. canis and 4 were infected by other parasitic infection. the results for control group were negative. conclusion: all the patients with inflammatory eye diseases such as endophthalmitis, uveitis dcr, glaucoma and cataract were studied for toxocara infection in this research work were at risk. therefore, in contrast to the previous idea, all eye inflammatory diseases in ocular patients should be considered for toxocara antibodies in addition to uveitis.