Evaluation of Schistosoma haematobium Adult and Egg Antigens by ELISA in Diagnosis of Urinary Schistosomiasis

Background: the aim of this study was using elisa for detection of anti-schistosomahaematobium antibodies in both sera and urine of patients with urinary schistosomiasis.methods: thirty three sera and urine samples were collected from patients with acute schistosomiasisin diyala province, east of iraq in 2006. their diseases were confirmed by finding s.haematobium ova in urine examination. sera and urines of 10 healthy individuals as well as 5patients with hydatidosis and 5 patients with acute toxoplasmosis were examined as well. sampleswere examined for antibody detection by elisa method. the antigens used in this studywere egg and adult antigens.results: all positive samples (sera and urines) showed positivity by using egg antigen whereasthe negative control samples were negative; only two samples with hydatidosis were positivewith using serum sample whereas with urine sample only one sample was positive. in this study,the best sensitivity and specificity obtained when using urine and adult antigen.conclusion: antibody detection by using urine is a useful, simple, and sensitive method for diagnosisof schistosomiasis.