Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in Pqe-30 Expression Vector

Background: echinococcosis or hydatid disease is a zoonotic infection caused by larval(metacestode) stages of cestodes belonging to the genus echinococcus, family taeniidae. weaimed to subclone antigen b gene in pqe-30 plasmid, its expression, and purification.methods: we subcloned hi gene into pqe-30 expression vector. the recombinant vectorwas transformed into e. coli, m15 and mass cultured. the subcloned gene was expressed byiptg. subcloning of gene was confirmed by both pcr and enzyme digestion.results: production of recombinant protein was confirmed by sds-page. western blotanalysis was carried out by both his-tag monoclonal ab and human serum to estimate theexpressed protein in e. coli cells. recombinant protein was purified and its specificity wasproved by western blotting.conclusion: production of this recombinant protein can increase sensitivity and specificity inserological test (elisa).