genotyping of echinococcus granulosus isolates from human in khorasan province, north-eastern iran

Background: human hydatidosis is endemic in northeastern iran. the present study aimed to investigate molecular diversity of echinococcus granulosus isolates collected from human surgically. methods: sixty human hydatid cysts (58 lung cysts and 2 liver cysts) were collected through surgery from ghaem and emam reza hospitals in mashhad university of medical sciences during 2015-2016. cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (pcrrflp) analysis of the internal transcribed spacer 1 (its1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1) and nadh dehydrogenase subunit i (nad1). results: overall, 55 out of 60 echinococcus granulosus cysts (91.6%) were determined as the g1 strain, 4 cases (6.6%) were determined as the g6 strain and 1 sample was not identified. conclusion: although sheep strain (g1) is dominated in human patients in great khorasan, the prevention of camel-dog cycle should pay attention in this region.