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molecular detection and genotyping of intestinal microsporidia from stray dogs in iran
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نویسنده
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delrobaei moein ,jamshidi shahram ,shayan parviz ,ebrahimzade elahe ,ashrafi tamai iraj ,rezaeian mostafa ,mirjalali hamed
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منبع
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iranian journal of parasitology - 2019 - دوره : 14 - شماره : 1 - صفحه:159 -166
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چکیده
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Background: microsporidia as one of the most important pathogens in veterinary and agricultural settings, have emerged in immunocompromised patients in iran. to date, different enterocytozoon bieneusi genotypes have been identified in humans and animals, supporting the possibility of zoonotic zoonosis transmission potential. the aim of this study was to evaluate the distribution of e. bieneusi genotypes among overpopulated stray dogs in vicinity of tehran, the capital city of iran. methods: totally, 75 stool and 75 urine samples were obtained from 75 stray dogs during the time period from mar 2015 to oct 2015. dna extraction was performed on all the samples and specific fragment of small subunit ribosomal rna gene of e. bieneusi and encephalitozoon spp. was amplified. furthermore, specific primers targeting the internal transcribed spacer region of e. bieneusi were applied to determine the genotype of the microorganism. results: microsporidia was detected in 5.3% of stool samples, while none of the urine samples was positive for microsporidia species. overall, 440 bp fragment of e. bieneusi was amplified in all the samples and there was no amplification for encephalitozoon spp. the results of sequencing of 410 bp fragment of internal transcribed spacer region showed that all the e. bieneusi were genotype d. conclusion: e. bieneusi was the most prevalent microsporidian species in the stray dogs and all the positive isolates were characterized as genotype d.
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کلیدواژه
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stray dogs ,microsporidia ,enterocytozoon bieneusi ,genotyping ,iran
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آدرس
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university of tehran, school of veterinary medicine, department of internal medicine, ایران, university of tehran, school of veterinary medicine, department of internal medicine, ایران, university of tehran, school of veterinary medicine, department of parasitology, ایران, university of tehran, school of veterinary medicine, department of parasitology, ایران, university of tehran, school of veterinary medicine, department of microbiology and immunology, ایران, tehran university of medical sciences, school of public health, department of medical parasitology and mycology, ایران, shahid beheshti university of medical sciences, research institute for gastroenterology and liver diseases, foodborne and waterborne diseases research center, ایران
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Authors
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