Gene Cloning of Iranian Leishmania major Mannose-1-Phosphate Guanyltransferase

Background: leishmania is an obligatory intracellular protozoan parasite, which infects human beingswhen infected sand fly vector takes a blood meal. most efforts are towards designing an effectivevaccine to prevent leishmaniasis. in this way, development of candidate antigen for vaccine has specialimportant. in this study, we cloned mannose-1-phosphate guanyltransferase gene of iranian l.major in pet32a expression vector.methods: primers based on l. major mannose-1-phosphate guanyltransferase sequence gene was designedand synthesized. dna of leishmania promastigotes was extracted and pcr reaction was done.pcr product was cloned into ptz57r and sub cloned into pet32a expression vector.results: recombinant plasmid containing 1140 bp as l. major mannose-1-phosphate guanyltransferasegene was extracted and confirmed by restriction analysis. pcr product was sequenced and depositedto genbank. there were some differences in amino acid sequences between iranian l. majormannose-1-phosphate guanyltransferase and others previously accepted in genbankconclusion: we amplified and cloned iranian l. major mannose-1-phosphate guanyltransferase successfully.