Immunoreactivity Analysis of Toxoplasma Gondii Recombinant Antigen Rsag3 in Sera From Immunized Balb/C Mice and Toxoplasmosis Patients

Background: the coccidian protozoa toxoplasma gondii is an obligate intracellular parasite of humans and other warm-blooded animals. diagnosis of toxoplasmosis is of considerable medical importance for human, especially pregnant women and immunocompromised individuals. the apply of an escherichia coli recombinant antigen(s) would be signifi-cantly useful in developing standardization of the diagnostic tests and reducing their costs. in this study, immunoreac-tivity of recombinant sag3 against sera from immunized mice and human anti-t. gondii igg positive patients was evaluated by western-blotting and enzyme immunoassay (eia) in department of parasitology and mycology, school of medicine, shiraz university of medical sciences in 2013. methods: three inbreed balb/c female mice were obtained. two mice were injected with rsag3 and one was re-mained untreated, as control. sera from immunized mice and also pooled sera from igg positive toxoplasmosis cases were evaluated with western-blotting. igg antibody responses to recombinant sag3 was measured by indirect elisa against the negative control group. results: the rsag3 protein reacted with sera of immunized mice and sera from patients with anti-toxoplasma igg antibodies in western-blot analysis. the result of elisa showed that, there was marked differences in the absorbance values between the recombinant sag3 immunized mice and control group. conclusion: the rsag3 showed igg reactivity with sera from immunized mice and anti-toxoplasma igg patients.