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molecular characterization of animal fasciola spp. isolates from kermanshah, western iran
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نویسنده
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bozorgomid arezoo ,nazari naser ,rahimi hamzeh ,beigom kia eshrat ,hajjaran homa ,mohebali mehdi ,aryaeipour mojgan ,heidarian peyman ,ezati mohammad saeid ,rokni mohamad bagher
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منبع
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iranian journal of public health - 2016 - دوره : 45 - شماره : 10 - صفحه:1315 -1321
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چکیده
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Background: we evaluated the genetic diversity of samples identified morphologically as fasciola spp. from sheep, cattle and goat from kermanshah province, western iran using pcr-rflp method. methods: we used pcr-rflp analysis of ribosomal its1 fragment using rsai restriction enzyme to investigate the genetic characteristics of fasciola species obtained from different hosts (16 sheep, 28 cattle, 4 goats). the species of fasciola were confirmed by sequencing the 700 bp region of ribosomal its1 gene. results: in kermanshah, f. hepatica was present in 96% of the samples, f. gigantica was found only in two cattle sample. no hybrid forms were detected in the present study. conclusion: our results contribute to clarify the dark spots of fasciola genotyping in different parts of iran.
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کلیدواژه
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fasciola hepatica ,fasciola gigantica ,genotype ,pcr ,iran
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آدرس
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tehran university of medical sciences, school of public health, dept of medical parasitology and mycology, ایران, kermanshah university of medical sciences, school of medicine, dept of medical parasitology and mycology, ایران, pasteur institute of iran, biotechnology research center, dept of molecular medicine, ایران, tehran university of medical sciences, school of public health, dept of medical parasitology and mycology, ایران, tehran university of medical sciences, school of public health, dept of medical parasitology and mycology, ایران, tehran university of medical sciences, school of public health, center for research of endemic parasites of iran (crepi), dept of medical parasitology and mycology, ایران, tehran university of medical sciences, school of public health, dept of medical parasitology and mycology, ایران, tehran university of medical sciences, school of public health, dept of medical parasitology and mycology, ایران, islamic azad university, science and research branch, faculty of veterinary medicine, dept of food hygiene, ایران, tehran university of medical sciences, school of public health, center for research of endemic parasites of iran (crepi), dept of medical parasitology and mycology, ایران
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پست الکترونیکی
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roknimoh@tums.ac.ir
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Authors
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