Simultaneous Detection of Caga and Cage of Helicobacter pylori Strains Recovered from Iranian Patients with Different Gastroduodenal Diseases

Background: to asses the status of two representative genes of cag pai i.e caga and cage of helicobacter pylori strainsinfecting iranian patients suffered from various clinical outcomes using one-step pcr.methods: a total of 120 h. pylori infected patients including non–ulcer dyspepsia, nud (n=81), peptic ulcer disease, pud(n=17), and gastric carcinoma, gc (n= 22) referred for endoscopy or gastric resection to amiralam hospital or cancerinstitute from 2005 to 2008 were assessed. the status of caga and cage genes was determined by gene specific pcr.results: 84.2% and 90.8% of the tested strains were positive for caga and cage, respectively. 81.7% strains were positivefor both caga and cage genes, whereas 8 (6.7%) were found double negative. the prevalence of caga in gc patients(100%) was slightly higher than pud patients (94.1%). all of gc cases were infected with caga-positive strains. the samedistribution pattern was indicated for cage gene in gc and pud patients. the caga-positive strains were significantlyassociated with gc as compared with nud (p< 0.05) but this association did not gain statistical significance when cagegene was assessed.conclusion: the concurrent detection of caga/cage genes allowed rapid and specific clarification of cag pai status. thestrains with caga/cage genotype are predominant in iran regardless of clinical outcome and create a distinct cluster patternfrom those in the west and similar to those of east asian countries. the current study also demonstrated that cage gene canbe explored as a better indication of cag-pai in iranian h. pylori strains.