Molecular Characterization of Subunit G of the Vacuolar ATPase in Pathogen Dermatophyte Trichophyton rubrum

Trichophyton rubrum is an anthropophil ic fungu s cau sing up to 90% of chronic cases of dermatophytosis, several propertiesof this fungus have been investigated so far. however, a few studies were carried out in the field of molecular biolo gy ofthis fungus. in the present study , we tried to identify the subunit g of its vacuolar atpase (v-atp ase). pairs of 21 ntprimers were designed from highly con serv ed region s of the v-atpase subunit g genes in other fungi . mentioned primerswere utilized in pcr using isolated genomic dna template as well as cytopl asmic rna of 'lrubrum and the pcr and rtpcrfragments were then sequenced. about 469 nucleotides were sequenced which encoded a polypeptide with 119 aminoac ids. nucleotide sequen ce comp arison in gene data banks (ncbi, nih) for both the dna and its dedu ced amino acidsequence revealed significant homology with v-atp ase subunit g genes and proteins of other eukaryotic cells . the aminoacid sequence of the encoded protein was about 84% identic al to the sequence of v-atpase subunit g from oth er fungi. insummary, we have cloned the first v-atpas e subunit g of dermatophytes and characterized it as a member of this genefamily in other eukaryotic cells.