A New Primer Pair in ITSl Region for Molecular Studies on Echinococcus granulosus

Background: echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having ahigh degree of genetic divergence. the strains characterization seems to be essential for the establishment of a preventiveand control strategy in every endemic area. using dna based methods for strain /genotype characterizations of e. granu/osushave some difficulties, especially access to an efficient and pure concentration of dna and proper primers. methods:using grinder method , a pure and high concentration dna was extracted from 10 human hydatid cysts collected from isfahan(central iran) hospitals, and processed for per reaction. results: using dnasis, the primers were designed in internaltranscribed spacer 1 (its i) region, following analysis of 30 e. granu/osus nucleotide sequences, extracted from gene bank.conclusion: thi s new and specific e. granulosus primer which amplified dna thoroughly can be applied for molecularstudies on echinococcosis.