Characterization of Specific Ige Antibody Related To Antigen 5 of Echinococcus Granulosus

Background: anaphylactic reactions, such as urt icaria , edema, respiratory symptoms, and anaphylactic shock often complicatethe course of cystic echinococcosis (ce). methods: to investigate the ral e of the ige immunoreactive antigen 5(ag 5) in the sera -positive patients with ce, we determined n-terminal of 57 kda subunit of ag5 responsible for ige andc-terrninal of this act ive antigen related to induction of igg specifically. results: immunoblotting analysis showed thatspec ific ige to 57-kda subunit related to inter-chain disulphide band of two 22 kda and 38-kda component of ag5 andconformational epitope on this subunits. in addition, since the 57 kda component arise fram the removal of the c-terminalportion of 22 kda subunit of ag5, thus ige spec ifically recognized n-terminal of 22 kda subunit which remain bound s tothe other component, whereas igg reacted with c-terminal of 38 kda component of ag5. conclusion: recognition of thespecifi c binding site on the 57 kda subun it of ag5 could leads to understanding the mechanism regul ating ige/lgg productionin some immune circumstances that ige tends to some dominate, whereas in other igg predominates.