Production of Antibody Raised Against Lipopolysaccharide (Lps) of Vibrio Cholerae Non-O1

Background: cholera, an infectious disease caused by vibrio cholerae, is primarily transmitted by ingestion of contaminatedfood or water. in severe cases, cholera may lead to severe dehydration, metabolic acidosis, and ultimately, hypovolemicshock and death. ethods: in this study v.cholerae non-o1 was cultured in suitable media. lps was extracted from the surface of bacteria by hot phenol-water method and then purified by high-speed centrifugation. for production of specific antibody against lps, white newzeland rabbits were first immunized by whole cell bacteria and then immunized with highly purified lps. the titre of the antiserum was determined by elisa for each srogroup.results: results presented in this study indicate that serum anti-lps antibodies raised against purified lps of v.choleraenon-o1 can detect v.cholerae non-o1. conclusion: this antibody had low cross reactivity with v.cholerae o1, serotype inaba or ogawa. so, this antibody can use for detection of v. cholerae non-o1.