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Identification of Entamoeba Histolyticaby Molecular Method in Surface Water of Rasht City,Iran
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نویسنده
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Hemmati A. ,Hooshmand E. ,Hosseini M.J.
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منبع
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Iranian Journal Of Public Health - 2015 - دوره : 44 - شماره : 2 - صفحه:238 -243
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چکیده
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Background: this study was conducted with the aim of determining surface water contamination with cysts of enta-moeba histolytica using pcr in rasht city,northern iran. methods: in this cross-sectional study,49 water samples including 18 rivers and 6 wetlands were collected from dif-ferent regions near the city of rasht in autumn of 2012. after filtration using 0.22 μm nitrate cellulose membrane fil-ters,the samples were examined using microscope and pcr method. results: in microscopic examination,four samples of the 49 samples were positive for cysts of e. (histolytica / dispar / muschkovskii). by using pcr method and molecular analysis,one sample was positive for e. histolytica. conclusion: in the molecular analysis,contamination by e. histolytica was proved in the waters of rasht city. further investigations including more samples and necessary preparations must be applied to prevent contamination. © 2015,iranian journal of public health. all rights reserved.
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کلیدواژه
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Entamoeba Histolytica; Iran; Pcr; Surface Water
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آدرس
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Dep. Of Microbiology,Rasht Branch,Islamic Azad University, ایران, Dep. Of Veterinary,Rasht Branch,Islamic Azad University, ایران, Molecular Biology Research Center,School Of Medicine,Baqiyatallah University, ایران
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Authors
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