Activity assay of glutathione S-Transferase (GSTs) enzyme as a diagnostic biomarker for liver Hydatid cyst in vitro

Background: the aim of this study was to detect the glutathione s-transferase(gst) enzyme activity of healthy/cystic liver as a diagnostic biomarker for hydatidosis. in order to compare with liver tissue,the level of the gsts enzyme activity of parasite was also determined. methods: parasites were collected from sheep liver tissue with hydatid cysts at a local abattoir and washed with pbs buffer. collected parasites and liver tissues were sonicated or homogenized respectively. extract solution samples were centrifuged and stored at - 20°c. gstenzyme activities were measured in the extract of parasite and liver tissue samples (healthy and infected livers). protein amounts and protein bands were detected using bradford and sodium do-decyl sulfate polyacrylamide gel electrophoresis (sds-page) methods respectively. to determine significant difference between two groups,two-samplet-test was performed. results: gst specific activities of healthy/infected livers and parasites were estimated 304,1297 and 146 u/ml/mgrespectively. significant highergst specific activities in cystic liver than healthy liver was observed (p<0.05). t-test analysis showed gst activity of parasite was lower than healthy liver tissue. sds-page showed gst protein bands with 24 kda in parasite samples and25 kda in liver tissues. conclusion: gst activity incystic liver tissue could be concerned as a biomarker for hydatid cyst diagnosis with other hydatid disease parameters.