Effect of miRNA-218-5p on Proliferation, Migration, Apoptosis and Inflammation of Vascular Smooth Muscle Cells in Abdominal Aortic Aneurysm and Extracellular Matrix Protein

Background: to explore the effects of mirna-218-5p on inflammation and extracellular matrix proteins of vascular smooth muscle cell line in abdominal aortic aneurysm (aaa). methods: mir-218-5p expression was detected with rt-qpcr. the proliferative activity of vascular smooth muscle cells (vsmcs) was detected with cck-8, the migration was detected by transwell, and the apoptosis was detected with flow cytometry. the expression levels of inflammatory factors (il-1β and il-18) were de-tected by elisa. the expression levels of proteins (mmp-9 and netrin-1) and adamts5 were detected by western blot. the targeting relationship between mir-218-5p and adamts5 was verified with dual-luciferase reporter assay. results: up-regulating mir-218-5p could significantly inhibit the proliferation and migration of vsmcs and induced the apoptosis (p<0.05). down-regulating mir-218-5p could significantly promote the proliferation and migration of vsmcs and inhibit the apoptosis (p<0.05). up-regulating mir-218-5p could inhibit the ex-pression levels of thp-1 cytoinflammatory factors (il-8 and il-1β), mmp-9 and netrin-1. adamts5 was the target gene of mir-218-5p. when there were both overexpression of adamts5 and upregulation of mir-218-5p, the upregulation of mir-218-5p could alleviate the effects of overexpression of adamts5 on the proliferation, migration and apoptosis of vsmcs. conclusion: mir-218-5p/adamts-5 molecular axis regulates the proliferation, migration, and apoptosis of vsmcs, as well as the expression of thp-1 cell inflammatory molecules and extracellular matrix molecules.